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Abstract
This study evaluated the effect of chitin depolymer (α-chitin) on hydrochloric acid, gamma irradiation and chitinase based on oligochitin A (1-3 kDa) and B (<1 kDa) by ultrafiltration membrane. Besides, the study evaluated the antioxidant activity by DPPH radical scavenging, lipid oxidation and antibacterial activity against the target bacterial strains by agar, MIC of oligochitin fractions receive. The results showed that oligochitin A and B have both antioxidant activity and inhibitory activity against the target bacterium, but oligochitin A activity is much stronger than that of oligochitin B. The oligochitin A fraction received the highest gamma irradiation (80.5%), the lowest was HCl (9.8%). This study showed that the depolymer chitin gamma irradiation (Co-60, 250 kGy) gave the highest (oligochitin A fraction) yield.
Keywords: chitin, oligochitins, chitin depolymer, gamma irradiation