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Abstract
Evaluation of the protective of dietary chitin supplementation to Vibrio parahaemolyticus in white leg shrimp Litopenaeus vannamei was performed with 6 treatments. Shrimp (mean weight 1.74 ± 0.22g) were fed diets supplemented with chitin at concentrations of 0%, 0.1%, 1%, 2%, 4% and 8% in triplicate for 2 weeks. Shrimp in all treatments were challenged by adding V. parahaemolyticus at the dose of 104,54cfu/mL directly to the culture tank. The experimental results showed that the cumulative mortality of the white leg shrimp in the treatment fed with 1% and 2% chitin supplement were 25% lower (P = 0.002) than the cumulative mortality of shrimp in the control treatment. There was no difference in cumulative mortality of white shrimp in the remaining treatments compared with control treatment (P>0.05).
Key words: Litopenaeus vannamei, chitin size, haemocyte, phagocytic activity.