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Abstract
The objective of the present study was to assess the effects of time after stimulation with domperidone (DOM), luteinizing hormone releasing hormone analog (LHRHa) and human chorionic gonadotropin (HCG) on biochemical features of seminal plasma in Waigieu seaperch Psammoperca waigiensis over period 72h post injection. Male broodfishes were injected pectoral fin corner with either 0.9% saline solution (control) or a single dose of DOM (20 mg/kg body weight (BW)) or LHRHa (20, 50, or 80 μg/kg BW) or HCG (500, 1000, or 1500 IU/kg BW). Milt samples were collected before hormone induction (0 h) to assess original milt properties condition, and at 24 h, 48 h, and 72 h post injection (p.i.) to examine the effects of hormone treatments on aspects of spermiation. Results showed that the treatment with saline solution or DOM or LHRHa did not significantly alter seminal plasma biochemical properties.
Keywords: Waigieu seaperch, hormonal stimulation, biochemical features, LHRHa, HCG, DOM