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Abstract

     To determine infection status and antibiotic resistance level of Vibrio spp. on white leg shrimp, the study has isolated all 240 strains from pond cultured shrimp samples and collected water samples in the provinces of Long An, Ben Tre, Bac Lieu, HCM city, and shrimp samples collected in Bac Lieu, Ninh Thuan. In addition, there were also samples of wild-caught shrimp collected at Dong Hai estuary (Bac Lieu). All of these strains were tested for antibiotic resistance by diffusion plate method with 24 antibiotics. Survey results show that among 240 strains of Vibrio spp., 100% of them were resistant to 1 type of antibiotics, 95% was resistant to 4 types of antibiotic, over 50% of bacterial strains were resistant to over 10 types of antibiotics, including 1 resistant to 21 types of antibiotic tested. Regarding multi-antibiotic resistance, none of the strains were sensitive to 24 tested antibiotics. The MAR index of bacteria isolated from shrimp post larvae in Ninh Thuan was very high (0.519). This showed that the Vibrio strains in this area was heavily exposed to antibiotics, resulted in antibiotic resistance. For commercial shrimp farms in Bac Lieu, the MAR index was 0.487, which suggested that shrimp farmer in the surveyed area still heavily relied on antibiotics during the farming process.


Keywords: Antibiotic resistance, white leg shrimp (Litopenaeus vannamei), Vibrio spp.

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Author Biographies

Hồ Khánh Duy

Khoa Thủy sản, Trường Đại học Nông Lâm Tp. Hồ Chí Minh

Truyện Nhã Định Huệ

Khoa Thủy sản, Trường Đại học Nông Lâm Tp. Hồ Chí Minh

Lưu Thị Thanh Trúc

Khoa Thủy sản, Trường Đại học Nông Lâm Tp. Hồ Chí Minh