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Abstract
The objective of the present study was to evaluate the effect of antibiotics on chilled storage sperm motility of grass carp (Ctenopharyngodon idella). The extenders were used in this study were HBSS (Hanks’ balanced salt solution), Modified HBSS, CCSE-2 (common carp sperm extender), Kurokuda-1 and Kurokuda-2. The dilution ratios were 1:1, 1:3 and 1:5 (sperm: extender). Two antibiotics Cephalexin and Amoxcelin were used in this study at the concentration of 50, 100 or 150 ppm. The experiments were conducted in a refrigerator at the temperature of 4ºC. The results showed that the sperm motility was the highest and activated to day 9 when Kurokuda-2 was used as the extender at the dilution ratio of 1:3. The sperm motility can be maintained until day 13 by adding 25ppm Cephalexin combined with 25ppm Amoxceline to extender.
Keywords: Grass carp, Ctenopharyngodon idella, sperm, chilled storage, extender, antibiotic.