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Abstract
This study were conducted to separate protein extracted marine sponge Ircinia mutans into fractions by a method of fractional precipitation with ammonium sulfate and evaluate antioxidant activity of the fractions through DPPH free radical scavenging activity and total reducing power. The results shown that fractional precipitation with 20 – 40% saturated ammonium sulfate recovered more than 99% of protein in the marine sponge extract. The protein with high antioxidant activity was obtained by reprecipitation of the fractions with 20% and 30% saturated ammonium sulfate.
Keywords: Marine sponge, protein, radical scavenging activity, total reducing power, fractional precipitation.
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Section
Articles