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Abstract
The purpose of this research was to use red meat of striped tuna (Sarda orientalis) as substrate to obtain protein hydrolyzate by hydrolysis reaction with NaOH as catalyst. Red meat of striped tuna was a perfect material to produce protein hydrolyzate with 22.42 ± 0.26% of protein content which is higher than other aquatic materials and by-products. The suitable hydrolysis reaction conditions were determined: 0.45 M of NaOH, 1:18 (w:v) of substrate weight to NaOH volume, 50 min of reaction time and 30°C of reaction temperature. Protein recovery yield reached a maximal value of 73.32 ± 1.29% at the suitable hydrolysis reaction conditions. This research has provided important information for applying NaOH as a catalyst to hydrolyzate aquatic materials and by-products to obtain protein hydrolyzate.
Key words: Red meat of striped tuna, hydrolysis reaction, NaOH as a catalyst, protein recover yield.