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Abstract
This study was carried out to evaluate the effects of anesthetic isoeugenol on water quality and survival rate of Asian seabass (Lates calcarifer) juvenile during open transportation in cool water. The experiment included four treatments of different isoeugenol-50% doses: 4 ppm (NT1), 6 ppm (NT2), 8 ppm (NT3) and 10 ppm (NT4). The fish with an average weight of 20.35 ± 0.15g were transported in aerated and oxygenated heatinsulating closed boxes with a density of 100 ind./40 L of water. Water quality, glucose concentration in blood and survival rate of the fish were recorded at beginning, after transport 6h and 12h, and 3 and 7 days after the end of transporting. The results showed that the water quality was declined expressed by decreased DO and pH, and increased CO2, TAN and NO2 during transportation but still in suitable ranges for asian seabass fingerlings. The blood glucose content of fish increased during transportation due to stress. The survival rates of the fish of all treatments were reduced following transporting time. The dose of isoeugenol was the most appropiate for open transportation of asian seabass fingerlings was 22°C during 12h with 6 ppm.