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Abstract
This study aimed to assess the impact of enriching live food with DHA Selco (DHA Protein Selco and A1 DHA Selco) on the growth, survival and metamorphosis rate of maroon clownfish larvae (Premnas biaculeatus). Five different concentrations of DHA Selco (50, 100, 150, 200, and 250 ppm) alongside a control group without enrichment were tested. The newly hatched maroon clownfish larvae were reared in 30-litre glass tanks at a density of one larva per litre. Rotifers were supplied to the rearing tanks for the first five days, while Artemia were provided from the third day until the end of the experiment. Each treatment was conducted with 3 replicates over a period of 45 days. The results revealed that enriching live food with DHA Selco significantly improved the growth, survival and metamorphosis rate of maroon clownfish larvae compared to the control group (P < 0.05). Overall, the measured factors exhibited a positive relationship with the increasing concentrations of DHA Selco supplementation, with the best value determined within the range of 150 - 200 ppm. Increasing the enrichment level to 250 ppm did not yield further improvements and even resulted in a decline in larval performance. This study highlights the crucial role of supplementing live food with DHA Selco in the successful rearing of maroon clownfish larvae, and the optimal recommended concentration ranging from 150 - 200 ppm.