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Abstract
DHA and other polyunsaturated fatty acids play an important role in improving the larval performance of marine finfish in general. In the present study, different concentrations of livefeed - enriched DHA (50, 100, 150 and 200 mg/liter and control, 0 mg/liter) were tested to evaluate the effect of this ingredient on growth, survival and metamorphosis of orange clownfish larvae. Larvae were reared in 30-liter glass tanks at a density of 1 fish/1.5 liters of water. Larvae were fed livefeed - enriched with DHA (A1 DHA Selco), consisting of rotifers (first 3 days) and metanauplius Artemia (from day 2 onwards). All treatements were conducted with three replicates for a period of 45 days. The study results showed that the enrichment of livefeed with DHA at any concentration (50 - 200 mg/liter) significantly improved the growth, survival and metamorphosis rates of orange clownfish larvae compared with the control (P < 0.05). In which, the supplement content of 150 mg/liter was determined to be optimal for this fish. Increasing the concentration to 200 mg/liter not only did not improve but also reduced the rearing results compared to the level of 150 mg/liter. From this study, it can be concluded that orange clownfish larvae should be fed livefeed - enriched DHA at the concentration of 150 mg/liter to achieve optimal growth, survival and metamorphosis rates.