Đánh giá hoạt lực tinh trùng hầu thái bình dương (<i>Crassostrea gigas</i> Thunberg, 1973) thông qua các thời điểm thu mẫu khác nhau

Abstract

     The objective of this study was to determine the time of sampling for the Pacific Oyster sperm (TBD) motility. The experiment was conducted at the end of March (L1), mid April (L2) and late April (L3). Evaluate activity by determining dilution ratio (1:50, 1: 100, 1: 150, 1: 200 sperm: artificial seawater); Osmotic pressure (300, 400, 500, 600 mOsm/kg); Cation Na⁺, Ca²⁺, K⁺, Mg²⁺ (cation concentrations are 0.2, 0.4, 0.6, 0.8M respectively) at the time of sampling L1, L2 and L3. Sperm motility was assessed through parameters of percentage and duration of sperm motility. The results showed that spermatozoa spermatozoa were best when diluted at 1: 200 at L3, ASTT at 500 mOsm/kg at L3, Na⁺ =0.4M, Ca²⁺ =0.2M, Mg²⁺ =0.4M at L3 and K⁺ = 0.4M at L2. The highest fertilization rates for artificial insemination in artificial seawater are lower than in treated natural sea water, followed by a 500 mOsm/kg osmotic pressure solution and the lowest in Na⁺= 0.4M solution with the result of 75.77 ± 5.26% respectively; 71.78 ± 3.25%; 49.94 ± 2.12%; 35.8 ± 5.27%.

Key words: Pacific oysters, dilution ratio, osmolatily, cation concentration, sperm motility