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Abstract
Gamma irradiation can be used as natural chitin cleavage based on irradiation effects of gamma rays. This is a relatively clean method of producing oligochitin. The method can be applied to produce oligochitin in industrial scale with large quantities. In this study, we optimized the production by surface-response method according to Box-Behnken model of chitin cleavage by gamma irradiation to oligochitin. The study has optimized the production stage of oligochitin A: 1÷3 kDa by suspension of gamma chitin suspension in 10% HCl acid solution at absorbed dose rate of 2.97 kGy/hour, absorbed dose 227.2 kGy and suspension chitin concentration in 10% HCl solution was 9.84%. In the optimal mode, the amount of oligochitin A fraction obtained was 64.41%. The oligochitin A fraction obtained had an antioxidant activity of 72.9÷89.4% compared with ascorbic and α-tocopherol acids and the resistance of tested strains (MIC value 250÷400 μg/ml).
Keywords: chitin, oligochitin, gamma irradiation.