Thu nhận và xác định tính chất của hydroxyapatit kích thước nano từ xương cá: (2) Sử dụng enzym cho quá trình tiền xử lý

Abstract

     Hydroxyapatite (HA) is a main component of human bone and teeth. In this study, HA was prepared from three fish bones including Barramundi (Lates calcarifer), Red Tilapia (Oreochoromis sp.) and Tilapia (Oreochoromis niloticus) by using an enzyme (Alcalase) for pre-treatment of fish by-products. The results showed that the reaction time could be reduced by pretreatment using enzyme. The TEM images and XPS spectra indicate that HA samples from Red Tilapia bones treated with Alcalase at 70ºC for 10 h and calcined at 700ºC for 60 min have a high crystallinity and porosity with a particle size of 30 – 50 nm. This process can be applied for obtaining raw fish bones as well as protein hydrolysate and lipid from fish by-products forward a zero-waste approach. It is also a potential process for large-scale production.

Keywords: Nanohydroxyapatite, fish bones, enzyme treatment methods, seafood waste