Ảnh hưởng của nồng độ các cation lên hoạt lực tinh trùng cầu gai (Tripneustes gratilla)

Abstract

    The objective of the present study was to assess the effects of cations on sperm motility of sea urchin Tripneustes gratilla (Linnaeus, 1758). Sperm was diluted in medium that contained cations as K⁺, Na⁺, Ca²⁺ and Mg²⁺ with the concentration of each cation was 0.2M; 0.4M; 0.6M and 0.8M at the ratio of 1:50 (semen: medium). Each treatment was replicated nine times. The result showed that sperm motility of sea urchin was the best at the cation concentration of K⁺/Na⁺/Ca²⁺ or Mg²⁺ at 0.4M/0.2M/0.8M or 0.2M, respectively. In conclusion, using K⁺ (0.4M), Na⁺ (0.2M), Ca²⁺ (0.8M) and Mg²⁺ (0.2M) as an artificial insemination medium/activation medium was effective for fertilizing/motility of sea urchin.