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Abstract
Collagen is widely used in medicine, pharmacy, cosmetics, and food. However, most published research focused on the preparation of collagen hydrolysis. This study presented a new procedure to prepare collagen flakes from barramundi scales (Lates calcarifer) before hydrolyzing them into collagen hydrolysate. The results indicated that collagen flakes had a high purity (mineral content <1%). Besides, The FTIR spectrum showed that all characteristic collagen peaks were unchanged after hydrolysis. Furthermore, the SDS-PAGE result showed that the Mw of collagen hydrolysis (about 10 kDa) was relatively small. In addition, from chemical composition analysis, collagen hydrolysis had a high purity and contained 17 amino acids. The findings in this study suggest a potential to obtain good-quality collagen flakes and hydrolysate from barramundi scales for application in such fields as cosmetics, food, and medicine.