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Abstract
Application of probiotics in improving water quality and controlling certain bacterial infection in shrimp are potentially less disease outbreaks. The present study was conducted to determine the inhibitory effects of Bacillus and Streptomyces on pathogenic Vibrio parahaemolyticus infection in white shrimp (Litopenaeus vannamei). It was found that addition of 105 CFU/mL of Bacillus and Streptomyces twice per week resulted in higher shrimp survival compared to that of positive control and relative percentage survival (RPS) was above 70% after 10 days of challenging shrimp with V. parahaemolyticus in vivo test. Moreover, the same study was applied in larger scale at farm level (600-700 m²) where white-leg shrimp were cultured for 100 days in pond in Soc Trang province. Adding probiotic formulations include Bacillus and Streptomyces twice per week could control V. parahaemolyticus. Furthermore, concentration of nitrite, ammonia slightly improve during 100 days in all experimental groups but those parameters were under permitted code QCVN 02-19:2014/BNNPTNT. On the other hand, the control pond with commercial probiotic was early harvested at 45 cultured days after stocking because of AHPND.
Key word: Bacillus, Streptomyces, AHPND, RPS (%)
Application of probiotics in improving water quality and controlling certain bacterial infection in shrimp are potentially less disease outbreaks. The present study was conducted to determine the inhibitory effects of Bacillus and Streptomyces on pathogenic Vibrio parahaemolyticus infection in white shrimp (Litopenaeus vannamei). It was found that addition of 105 CFU/mL of Bacillus and Streptomyces twice per week resulted in higher shrimp survival compared to that of positive control and relative percentage survival (RPS) was above 70% after 10 days of challenging shrimp with V. parahaemolyticus in vivo test. Moreover, the same study was applied in larger scale at farm level (600-700 m²) where white-leg shrimp were cultured for 100 days in pond in Soc Trang province. Adding probiotic formulations include Bacillus and Streptomyces twice per week could control V. parahaemolyticus. Furthermore, concentration of nitrite, ammonia slightly improve during 100 days in all experimental groups but those parameters were under permitted code QCVN 02-19:2014/BNNPTNT. On the other hand, the control pond with commercial probiotic was early harvested at 45 cultured days after stocking because of AHPND.
Key word: Bacillus, Streptomyces, AHPND, RPS (%)