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Abstract
Effect of food types, exogenous hormone, temperature and salinity on captive breeding and embryonic development of grunting toadfish Allenbatrachus grunniens (Linnaeus, 1758) was conducted with three experiments. In the experiment 1, the effect of different food types on grow out broodstock spawning includes four treatments with 3 replicates, i.e., (i) Treatment 1: 70% crustacean, 20% trash fish, 10% squid; (ii) Treatment 2:: 50% crustacean and 50% trash fish; (iii) Treatment 3: 50% crustacean and 50%squid); (iv) Treatment 4: 100% UP pellets.. Experiment 2 was to determine the suitable hormone (LHRHa, HCG) dose used in spawning of grunting toadfish, consisting of 9 treatments. Experiment 3 was to examine the effect of temperature (28oC, 30oC, 32oC) and salinity (14‰, 17‰, 20‰, 23‰ and 26‰) on embryonic development of grunting toadfish. Results showed that feeding of use 50% crustacea and 50% fresh was the most suitable for broodstock. Combination of LHRHa with DOM and HCG at 50μg LHRHa + 5mg DOM +1000UI HCG kg-1 female fish resulted in good maturation. The highest hatching rate was obtained at 28oC and 20‰, This study provides the important background for captive breeding of this species.
Keywords: effect, eggs of incubation, grunting toadfish, spawning, grow out.