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Abstract
ABSTRACT
This study evaluates the effectiveness of chitosan-based bioflocculation for harvesting Nannochloropsis sp. biomass and the efficiency of pigment preservation in the microalgal biomass during storage. The microalgal biomass harvesting process was carried out using chitosan (molecular weight ~520 kDa, concentration 60 ppm) followed by cloth filtration. The results showed a biomass recovery efficiency of approximately 88%, while the recovery efficiencies of chlorophyll-a and carotenoids were approximately 95% and 89%, respectively. The concentrated microalgal biomass was supplemented with 0.1% vitamin C and stored at 4±2°C. After 10 weeks of storage, the loss rates of carotenoids and chlorophyll-a were approximately 7% and 5%, respectively. These results indicate the potential of the proposed harvesting and preservation method for maintaining the quality of pigment-rich Nannochloropsis sp. biomass.
Keywords: Chitosan, Nannochloropsis sp., biomass recovery efficiency, chlorophyll-a, carotenoid.